3D-Printed Microfluidic Artificial Lungs
Designed and manufactured various 3D-printed microfluidic artificial lungs.
Published Abstract: https://asaio.org/conference/program/2023/P44.cgi
Purpose:
Current hollow-fiber oxygenators used in Extracorporeal Membrane Oxygenation have suboptimal gas exchange performance and hemocompatibility. Microfluidic artificial lungs (µALs) promise smaller feature sizes, improving gas exchange efficiency, and biomimetic flow paths, improving blood compatibility. For the first time, we leverage the geometric freedom of 3D-printing technology to demonstrate µALs with truly 3D, biomimetic branching capillaries and gas-side designs to increase gas exchange surface area.
Methods:
Two µAL designs were generated in Solidworks and printed on an Asiga MAX X27 UV printer. The first was a "simple branching" µAL (Fig 1A) with quadfurcating, biomimetic branching for blood distribution. The second was a "hatched" µAL (Fig 1B) employing a gas phase with strategically placed supports to maximize gas exchange surface area while maintaining structural stability. These changes are only to the gas, and so the blood-phase is unchanged. Dyed water was used to visualize internal structures (Fig 1D). Geometric analysis and proven models were used to calculate theoretical shear rate and rated flow. Computational Fluid Dynamics (CFD) was used to visualize flow (Fig 1E,F). Experimental pressure drop vs water flow rates were measured (Fig 2).
Results:
Through geometric analysis (Table 1), branching distribution technique provides more uniform shear stress (0.95-1.34 dyn/cm2) compared to a non-branching "open-plenum" control (0.09-1.27 dyn/cm2). The hatched µAL has a 3.18 mL/min rated flow, 1.7x greater than the 1.84 mL/min rated flow of the non-hatched branching µAL. As a result of the higher rated flow, it also has higher theoretical shear of 1.64-2.31 dyn/cm2 (at rated flow) despite identical blood-side geometry. The branching lung demonstrated a pressure drop up to 12 mmHg at flows up to 5 mL/min (Fig 2). These are significantly larger than analytically predicted values (extrapolated 7 mmHg vs 0.233 mmHg at 3.2 mL/min), prompting investigation into resolution of internal capillaries and effects of surface roughness on pressure drop.
Conclusion:
We have demonstrated the first 3D-printed branching biomimetic µAL formed from a gas permeable material, with gas-side geometries to improve gas exchange efficiency. These geometries are expected to translate to greater blood flows enabling creation of future animal and human-scale µALs.
